We bridge the gap between fundamental research on enzyme structure and function, and enzyme application.
Services
Our biocatalysis services harness the power of enzymes to drive transformative solutions across various industries. Whether you require end-to-end support or targeted expertise, our comprehensive offerings are designed to help you achieve your goals efficiently and effectively.
Enzyme scouting
We provide an enzyme scouting service tailored to your reaction and substrate of interest. Using extensive resources such as databases, literature, and advanced modeling techniques, we curate an enzyme panel specifically suited to your needs, followed by production and validation, often complemented with further enzyme and process optimization.
Small to medium-scale production
Whether it's an enzyme panel designed by us or specific sequences identified by the client through literature, databases, or in silico design, we provide extensive cloning and expression services for enzymes of all types. Our mutagenesis pipeline enables efficient cloning and expression of complex mutant libraries in MTP format. We handle in-house scaling up to gram-scale enzyme production and collaborate with trusted partners for larger quantities.
Enzyme testing
After cloning and expression, we perform enzymatic reaction setup and analyses using a range of methods, such as colorimetric assays, HPLC with DAD, CAD or MS detection, ensuring comprehensive analysis to assess the productivity, activity, kinetic performance, and stability of the enzymes. If enhancements are required in any area, we employ enzyme engineering or process optimization to achieve the desired improvements.
Process optimization
With extensive experience in optimizing processes for various enzyme classes, we have the expertise to determine optimal conditions for both stability and reactivity. This involves fine-tuning reaction parameters such as temperature, pH, substrate concentration, and enzyme dosage to maximize activity and stability. Additionally, it includes ensuring substrate specificity, incorporating necessary co-factors, and monitoring reactions in real-time, balancing all these factors to achieve optimal performance and yield